Biotechnology MCQs Question bank with Answers
1.Taq Enzyme utilized in PCR is
A) DNA without 3’ to 5 ‘proofreading exonuclease activity.
B) DNA with 3’ to 5 ‘proofreading exonuclease activity.
C)DNA without 5’ to 3’ proofreading exonuclease activity.
D)DNA with 5’ to 3’ proofreading exonuclease activity.
2.Shotgun method used in
A) DNA sequencing.
B) Gene mapping.
C)Gene transformation.
D)Genomic library creation.
3.Joining of donor DNA fragments and vector DNA fragments with the help of
DNA ligase enzyme is known as
A.Gene cloning
B..Splicing
C..Gene manipulation
D. Molecular cloning
4.Suitable host in genetic engineering to introduce DNA fragments of donor is
A. Yeast
B. Bacillus subtilis
C. Escherichia coli
D. Bacteriophages
5.The mechanism which has the ability to engineer new organisms is known as
A. Totipotency
B. Molecular cloning
C. Genetic engineering
D. Splicing
6.The extra-chromosomal circular DNA found in the E.coli is
A. Plasmid
B. DNA ligase
C. Vector
D. Cytokinin
7.If the sequence of bases in DNA is TAGC, then the sequence of bases in RNA
will be:
A.ATCG
B.TAGC
C.AUCG
D.CGAT
8.The enzyme that cleaves DNA at specific sites, producing sticky ends is called
A. Restriction endonuclease
B. Cleaving enzyme
C. Lysing enzyme
D. Exonuclease
9. Ti Plasmid naturally occurs in
A.Agro bacterium
B.Corynebacterium
C.Staphylococcus
D.Vibrio
10. A patient receives long-term, high-dose therapy with penicillin. After approximately 2weeks of therapy, the patient has a low-grade fever, rash, and
muscle andjoint pain. Which type of hypersensitivity accounts for these symptoms?
A.Type I
B.Type II
C.Type III
D.Type IV
11.Gene therapy
A.Is a technology that is widely used in patient care
B.involves short nucleic acid sequences to inhibit gene expression
C.has not been tested in clinical trials
D.All of the above
12.CD41 T cells specifically recognize antigens in which form?
A. Bound to major histocompatibility complex(MHC) class I molecules on the
surface of any nucleated cell In free, soluble form in extracellular fluids
B.Bound to MHC class II molecules on the surface of special antigen- presenting cells (APCs)
C.Bound to complement receptors on professional antigen-presenting cells (APCs)
D.Associated with class II MHC on the surface of TC cells.
13. Which primers are used in annealing during amplification of gene?
A.Reverse primers
B.Forward primers
C.Oligo nucleotide primer
D.Internal primers
14.Which molecule or part of a molecule is properly matched to its function?
A.DNApolymerase-‐-‐ transcription
B.RNApolymerase-‐ translation
C.amino-‐acylt RNA synthetase-‐-‐ DNA replication
D.peptidyltransferase-‐-‐ Okazakifragments
E.none of the above pairs are properly matched.
15.Which of the following is not a difference between RNA and DNA?
A.RNA has uracil and DNA has thymine.
B.RNA has ribose and DNA has deoxyribose.
C.RNA has 5 bases and DNA has 4.
D.RNA is a single polynucleotide strand and DNA is a double strand.
E.RNA is relatively smaller than human chromosomal DNA.
16. What would be the typical time frame for the appearance of a type IV hypersensitivity reaction in aperson already sensitized to the antigen?
A.Within a few minutes
B.Within a few hours
C.Within a few days
D.Within a few weeks
E.Within a few months
17. The technique involved in comparing the DNA components of two samples
is known as
A.Monoclonal antibody techniques
B. finger printing
C.Recombinant DNA technology
D.Polymerase chain reaction
18.Plasmids are ideal vectors for gene cloning as
AThey can be multiplied by culturing
B.They can be multiplied in the laboratory using enzymes
C.They can replicate freely outside the bacterial cell
D.They are self replicating within the bacterial cell
19.The main difference between a plasmid and an episome is:
A.Plasmid is circular episome is linear
B.Episome is circular, plasmid is linear
C.Plasmid can be insert into host genome, episomes are not
D.Episomes can be inserted into the host genome
20.In a antigen haptens are
A.Immunogenic
B.Non-immunogenic
C.Antigenic
D.none
21.The most important discovery that lead to the development of rDNA technology was
A.Discovery of Watson & crick helix DNA model
B.Discovery of restriction endonuclease
C.Discovery of ligase
D.Discovery of tissue culture cloning.
22.The group associated with first man made recombinant DNA molecules:
A.Daniel Nathans, Arber, Kary Mullis
B.Paul Berg, Annie Chang, Boyer, Stanley Cohen
C.Howard Temin, Sydney Brenner, Philip Sharp
D.Tim Hunt, Paul Nurse, Leyland Hartwell
23. A vector should have which of the following properties i) MCS ii) Small size
iii) Multiple ori iv)low replication speed
A.i, ii, iii
B.ii, iii,iv
C.i, ii, iv
D.i, iii, iv
24.The mechanism of intake of DNA fragments from the surrounding medium
by a cell iiv called
A.transformaon
B.transducon
C.both a and b
D.conjugaon
25. Which of the following is not a restriction endonuclease?
A.Eco RI
B.DNA ligase
C.Hind III
D.Bam H1
26. Taq polymerase is used in PCR because of its
A.low thermal stability
B.high fidelity
C.high speed
D.high thermal stability
27. Cell mediated immunity can be identified by –
A.Sheep bred blood corpuscles roasette formation
B.Microphase inhibiting factor
C.Skin test for delayed hyper sensitivity
D.all
28.The uptake of plasmid DNA into bacterial cell is facilitated in the presence of
A.calcium chloride
B.magnesium chloride
C.potassium chloride
D.all of these
29. Use of alkaline phosphatase is to
A.remove terminal phosphates from 3’end
B.remove terminal phosphates from 5’end
C.remove terminal phosphate from both end
D.All of these
30.Which of the following enzyme is used to synthesize DNA using an mRNA
template
A.Taq polymerase
B.alkaline phosphatase
C.reverse transcriptase
D.nuclease
31.The enzyme that adds mononucleotide triphosphates tothe 3’ OH group of a DNA fragment is
A.polynucleotide kinase
B.terminal nucleotidyl transferase
C.terminal phosphoryl transferase
D.all of these
32..Triple toxoid vaccine gives protection against
A..Diphtheria, tetanus and rabies
B.Tetanus, whooping cough, Tuberculosis
C.Whooping cough, tetanus and Diphtheria
D.Whooping cough, cancer and T.
33.First genetically engineered and biotechnologically produced vaccine was
against.
A.AIDS
B.Small pox
C.Herpes simplex
D.Hepatitis B
34. Genomic DNA is extracted, broken into fragments of reasonable size by a
restriction endonuclease and then inserted into a cloning vector to generate chimeric vectors. The cloned fragments are called
A.Clones
B.Genomic library
C.mRNA
D.none
35.In which medium the hydridoma cells grow selectively
A.Polyethylene glycol
B.Hypoxanthine aminopterin thyminine
C.Hypoxathing-guaning phosphoribosyl transferase
C.Both b and c
36.Joining of donor DNA fragments and vector DNA fragments with the help of
DNA ligase enzyme is known as
A.Gene cloning
B. Splicing
C. Gene manipulation
D. Molecular cloning
37.Suitable host in genetic engineering to introduce DNA fragments of donor is
A.Yeast
B. Bacillus subtilis
C. Escherichia coli
D. Bacteriophages
38.The mechanism which has the ability to engineer new organisms is known as
A.Totipotency
B. Molecular cloning
C. Genetic engineering
D.Splicing
39.The extra-chromosomal circular DNA found in the E.coli is
A.Plasmid
B. DNA ligase
C. Vector
D. Cytokinin
40.The production of new characteristics by introducing new genes and altering the genome is known as
A.DNA recombinant technology
B.Protoplast fusion
C. Totipotency
D. Splicing
41.The molecular scissors which cut DNA at specific sites are
A. Plasmids
B. Fusogenic agents
C. Inoculum
D: Restriction enzymes
42. An antibody probe is used in
- (A) Southern blotting
- (B) Northern blotting
- (C) Western blotting
- (D) None of these
43. The first protein synthesized by recombinant DNA technology was
(A) Streptokinase
(B) Human growth hormone
(C) Tissue plasminogen activator
(D) Human insulin
44. Monoclonal antibodies are prepared by cloning
- (A) Myeloma cells
- (B) Hybridoma cells
- (C) T-Lymphocytes
- (D) B-Lymphocytes
45. Trials for gene therapy in human beings were first carried out, with considerable success, in a genetic disease called
(A) Cystic fibrosis
(B) Thalassemia
(C) Adenosine deaminase deficiency
(D) Lesch-Nyhan syndrome
46. Which of the following may be used as a cloning vector?
(A) Prokaryotic plasmid
B) Lambda phage
(C) Cosmid
(D) All ssyndrome
47. DNA fragments upto 45 kilobases in size can be cloned in
(A) Bacterial plasmids
(B) Lambda phage
(C) Cosmids
(D) Yeast artificial chromosomes
48. Twenty cycles of PCR can amplify DNA:
(A) 220 fold
(B) 202 fold
(C) 20 x 2 fold
(D) 20 fold
49. DNA finger printing is based on the presence in DNA of
(A) Constant number of tandem repeats
(B) Varibale number of tandem repeats
(C) Non-repititive sequences in each DNA
(D) Introns in eukaryotic DNA
50. In the ’lac operon’ concept, which of the following is a protein?
- (A) Operator
- (B) Repressor
- (C) Inducer
- (D) Vector
51. A Gene is
(A) A single protein molecule
(B) A group of chromosomes
(C) An instruction for making a protein molecule
(D) A bit of DNA molecule
52. In DNA, genetic information is located in
(A) Purine bases
(B) Pyrimidine bases
(C) Purine and pyrimidine bases
(D) sugar
53. Myeloma cells and lymphocytes can be fused by using
- (A) Calcium chloride
- (B) Ethidium bromide
- (C) Polyethylene glycol
- (D) DNA polymerase
54. Diphtheria toxin inhibits
- (A) Prokaryotic EF-1
- (B) Prokaryotic EF-2
- (C) Eukaryotic EF-1
- (D) Eukaryotic EF-2
55.The main difference between a plasmid and an episome is:
A.Plasmid is circular episome is linear
B.Episome is circular, plasmid is linear
C.Plasmid can be insert into host genome, episomes are not
D.Episomes can be inserted into the host genome
56.The antiviral protein produced by virus infected cells is called
A. Insulin
B. Auxin
C. Vaccine
D. Interferon
57. The vaccines prepared through recombinant DNA technology are
a. Third generation vaccines
b. First-generation vaccines
c. Second-generation vaccines
d. None
58. The expression of a transgene in the target tissue is identified by a
a. Transgene
b. Promoter
c. Enhancer
d. Reporter
59. The DNA fragments have sticky ends due to
a. Endonuclease
b. Unpaired bases
c. Calcium ions
d. Free methylation
60. ELISA is
a. Using radiolabelled second antibody
b. Usage of RBCs
c. Using complement-mediated cell lysis
d. Addition of substrate that is converted into a coloured end product
