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Bioassay (Short Questions)

Bioassay  important Question For One Mark 

True or False

1) Bioassay is the method of estimating the potency of an active principle .
2) Interpolation Method of bioassay procedure involves the test substance and standard substance, and responses produced are compared with a trial and error proc edure till they generate equivalent effects.
3) Bioassay methods are used for the standardisation of drugs of synthe otircigin.
4) Bioassay methods are used for the selection of new compounds for determining their biological activity.
5) There are two major types of bioassays.
6) Standard insulin is pure, dry, and crystalline.

Fill in the Blanks

7) __________ of bioassay is the easiest graded response method.
8) _________ and ____________ articulated certain principles for conducting biological assays.
9) Dehydrated alcohol is used for extracting a particular amount of ____________ powder in a continuous extraction apparatus for 6 hours.
10) The bioassay of ____________ shows graded dose response and matching assay of histamine in the ileum of guinea pig.
11) The vasopressor activity o f _______________ is determined by comparing the activity of its test preparation with that of its standard preparation under the conditions of an appropriate method of assay.

Answers

1) True 2) False 3) False 4) True 5) False 6) True 7) Interpolation method 8) Burn, Dale 9) Digitalis 10) Histamine 11) Arginine vasopressin

Important Summary For one mark Question

1) Bioassay (or biological assay or biologic standardisation) is the method of estimating the potency of an active principle in a unit amount of formulation or detection and measurement of a substance’s concentration in a formulation using biological methods (i.e., observation of clinical effects on living tissues, micro/macro or immune cells or animal).
2) Burn and Dale articulated certain principles for conducting biological assays.
3) Matching Method of bioassay procedure involves the test substance and standard substance, and responses produced are compared with a trial and error procedure till they generate equivalent effects.
4) Interpolation Method of bioassay is the easiest graded response method, and does not need any statistical data or many calculations. Firstly, the drug – response curve is obtained from varying doses of standard ACh solution, and then concentration of unknown is read from the standard graph.
5) Bioassay methods are used for the standardisation of drugs of natural origin.
6) Bioassay methods are used for the selection of new compounds for determining their biological activity.
7) There are three major types of bioassays (apart from qualitative assays).
8) Standard insulin is pure, dry, and crysta lline. As stated by the Ministry of Health, Government of India, one unit consists of 0.04032mg of insulin, which is equal to international unit.
9) The vasopressor activity of arginine vasopressin is determined by comparing the activity of its test preparati on with that of its standard preparation under the conditions of an appropriate method of assay.
10) Bioassay of ACTH is an old assay method. Upon administration, ACTH decreases the ascorbic acid available in adrenals. This association is used for the quantitative assay of ACTH.
11) Dehydrated alcohol is used for extracting a particular amount of digitalis powder in a continuous extraction apparatus for 6 hours. The bioassay of histamine shows graded dose response and matching assay of histamine in the ileum of guinea pig.
12) Each dose of histamine, 5 -HT, adrenaline, noradrenaline, and isoprenaline are administered in terms of base, and all others in terms of salt. Krebs solution is gassed with 95% O2 + 5% CO2, whereas all others are gassed with O2

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