Biochemistry imp question bank

Question Bank
Biochemistry(BP-203)
Unit-1

Question Containing 2 Marks

1. Give definition of biomolecules.
2. Give definition of bioenergetics.
3. Define the following term
   a) Enthalpy
   b) Entropy
4. Classify protein on the basis of structure with examples
5. Define exothermic and endothermic reaction
6. Define activation energy.1
7. 1 NADH molecule equal to haw many molecules of ATP.
8. 1 GDP molecule equal to haw many molecule of ATP.
9. What is gibbs free energy.
10. What are globular proteins and give example
11. What are nucleic acids.

Question Containing 5 Marks

1. Explain the biological role of carbohydrates.
2. What are lipids classy them.
3. Write a short note on oxidative phosphorylation
4. Explain the biological role of lipds
5. Give energetic and significance of TCA cycle.
6. Give chemical and physical properties of carbohydrayes
7. Difference between endergonic and exergonic reation.

Questions containing 10 marks

1. Define Proteins and classify them.
2. What are carbohydrates and classify them.
3. Define glycolysis, give its significance and energetic.
4. Briefly explain about between free energy, enthalpy and entropy.
5. Give function of biomolecules.

Unit-2

Question Containing 2 Marks

1. Haw many no. of ATP used and formed in glycolysis pathway.
2. What do you understand by HMP shunt.
3. Haw no. of ATP used and formed in kerb cycle.
4. Write significance of HMP shunt pathway.
5. Expalin about glycogen storage diseases.
6. Define Inhibitors and uncouplers.
7. Where does glycolysis and krebs cycle occurs.

Question Containing 5 Marks

1. Explain about oxidative phosphorylation on substrate level.
2. Write a short on ETC and its inhibitors.
3. Define glycolysis and its pathway.
4. Define oxidative phosphorylation and its mechanism.
5. Define kreb cycle and its pathway.
6. Write a short note on inhibitors of oxidative phosphorylation.
7. Discuss the pathway of glycogen pathway
8. Explain about HMP shunt.
9. Write the pathway of gluconeogenesis.
10. Briefly explain glycogen metabolism and glycogen storage diseases.

Question Containing 10 Marks

1. Explain glycolysis pathway and its significance and energetic.
2. Briefly explain glycogen metabolism pathway and glycogen storage
   disease.
3. Describe krebs cycle and its significance and energetic.
4. Explain diabetes mellitus and its test.
5. Discuss about glycogen storage diseases and G6PD deficiency.
6. Write brief note on hormonal level of blood glucose level.
7. Explain about electron transport chain and write its mechanism.
8. Write brief note on HMP shunt and its significance.
9. Explain about gluconeogenesis pathway and its significane.

Unit-3
Question Containing 2 Marks

1. What do you understand by the term Transamination? Give example.
2. Write down the synthesis of 5-HT (5-hydroxytryptamine) from Tryptophan.
3. What is hyperbilirubinemia?
4. Define vitamin D?
5. Define cholesterol?
6. Briefly describe atherosclerosis.
7. What is jaundice?

Question Containing 5 Marks

1. Enumerate the ketone bodies. Describe the formation and utilization of ketone bodies in the body.
2. Discuss in detail about the De novo synthesis of fatty acids.
3. Write a note on conversion of cholesterol into bile acid and its role?
4. Write a short note on i)Tyrosinemia ii)Hypercholesterolemia
5. Discuss in detail about urea cycle. Also give the significance of urea cycle.
6. Write down the biosynthesis and biological significance of Dopamine, Noradrenaline and Adrenaline.

Question Containing 10 Marks

1. Write the biosynthesis of ketone bodies and their utilization?
2. Write a short note on i)Catabolism of heme ii)Albinism
3. Write a note on β-oxidation of saturated fatty acid.
4. What is deamination? Discuss the metabolic disorder of phenylalanine.
5. Write about disorders of lipids metabolism.

Unit-IV
Questions containing 2 marks

1. Define nucleic acids.
2. Name the enzyme associated with hyperurecimia.
3. Give name of the end product of purine metabolism.
4. Give name of the amino acid required for the synthesis of purines and pyrimidines.
5. Write a note on Chargaff’s rule.
6. Write a note on hydrogen bonds in DNA.

Questions containing 5 marks

1. Describe the structure of DNA.
2. Name different RNAs and discuss their structure.
3. Write an account structure, function and nomenclature of nucleotides.
4. Describe the structure of nitrogenous bases present in nucleic acids.
5. Define genetic code.
6. Write a short note on gout.
7. Define hyperuricemia.
8. Describe the catabolism of purine nucleotide.

Questions containing 10 marks

1. Explain about biosynthesis of purine nucleotide.
2. Briefly describe about protein synthesis and inhibitors.
3. Describe the role of PRPP in purine and pyrimidine synthesis
4. Write an account of biosynthesis of inosine monophosphate
5. Discuss the synthesis and degradation of pyrimidines.

Unit-V
Questions containing 2 marks

1. What do you mean by competitive inhibitors?
2. What is allosteric inhibition.
3. Which type of curve is obtained when substrate concentration is plotted against enzyme
   velocity?
4. Define Km. (Michaelis Menten Constant.)
5. Define isoenzyme with example.
6. Define enzyme.
7. Give an example of oxidireductases
8. Give the IUB classification of enzymes.
9. Define enzyme kinetics.
10. What are allosteric enzymes?
11. Define isoenzymes.
12. Define co enzyme.
13. Define enzyme induction.
14. Define enzyme repression.

Questions containing 5 marks

1. Define Enzyme and classify them.
2. Give the IUB classification of enzyme with example.
3. How the naming of the enzymes is done.
4. What do you mean by enzyme induction and repression.Give a detail account.
5. What do you mean by allosteric enzymes? Give examples.
6. Write a note on michaelis menten equation.
7. Write down the therapeutic and diagnostic application of enzymes
8. Write a note on the structure of coenzyme.

Questions containing 10 marks

1. Give the derivation of Michaelis Menten Equation and also explain factors affecting enzyme activity.
2. Write in detail the mechanism of inhibition of a reaction catalysed by an enzyme.
3. Derive Michaelis-Menten equation with their significance.
4. Define enzyme and classify them. Describe various factors affecting the rate of enzyme catalysed reactions.
5. Write in detail about Line Weaver Burke Plot and its significance.
6. What do you mean by competitive and non-competitive inhibitors.write about their kinetics using Line Weaver plot.
7. Define enzyme. Write about the nomenclature of the enzymes.
8. Define enzyme. Give the IUB classification of enzymes.
9. Write about enzyme inhibitors and their kinetics.
10. Define coenzyme.write a note on its structure.
11. Define enzyme and give the therapeutic and diagnostic applications of enzymes.
12. Write a note on the regulation of allosteric enzymes.