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Different Test For Amino Acid And Protein Detection

POINTS TO REMEMBER

DIFFERENT TEST FOR AMINO ACID AND PROTEIN DETECTION

NINHYDRIN REACTION – Alfa amino acid

 MILONS REACTION – Phenolic Amino acid

BIURET REACTION – Two peptide linkage

NITROPRUSSIDE REACTION, SUFER TEST – Sulfahydryl group (Cysteine)

PAULY’S TEST – Imidazole ring (Histidine)

FOLIN COICATEAU’S TEST – Phenolic group (Tyrosine)

SAKAGUCHI REACTION – Guanidino group (Arginine)

HOPKINS KOLE REACTION – Indole ring (Tryptophan)

XANTHOPROTENIC REACTION – Aromatic amino acid (Tyrosine, Tryptophan)

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