POINTS TO REMEMBER
DIFFERENT TEST FOR AMINO ACID AND PROTEIN DETECTION
NINHYDRIN REACTION – Alfa amino acid
 MILONS REACTION – Phenolic Amino acid
BIURET REACTION – Two peptide linkage
NITROPRUSSIDE REACTION, SUFER TEST – Sulfahydryl group (Cysteine)
PAULY’S TEST – Imidazole ring (Histidine)
FOLIN COICATEAU’S TEST – Phenolic group (Tyrosine)
SAKAGUCHI REACTION – Guanidino group (Arginine)
HOPKINS KOLE REACTION – Indole ring (Tryptophan)
XANTHOPROTENIC REACTION – Aromatic amino acid (Tyrosine, Tryptophan)
