MICROBIOLOGY-2
1. A peculiar cytochrome is observed in bacteria and it can react with molecular oxygen, what is it?
a. Cyt b
b. Cyt c
c. Cyt d
d. Cyt o
2. The genetic material in HIV is
a. ds DNA
b. ss DNA
c. s RNA
d. None of these
3. Which one of the following mutagens act only on replicating DNA?
a. Ethidium bromide
b. Nitrosogeranidine
c. Acridine orange
d. None of above
4. Poly A tail is frequently found in
a. Histone in RNA
b. Bacterial RNA
c. eukaryotic RNA
d. TRNA
5. Which of the following is an example of RNA virus?
a. SV 40
b. T4 phage
c. Tobacco mosaic virus
d. Adeno virus
6. Genomic DNA is extracted, broken into fragments of reasonable size by a restriction endonuclease and then inserted into a cloning vector to generate chimeric vectors. The cloned fragments are called
a. Clones
b. Genomic library
c. mRNA
d. None of these
7. Transgenic animals are produced when GH gene fused with
a. MT gene
b. GH
c. GRF
d. FIX
8. In which medium the hydridoma cells grow selectively?
a. Polyethylene glycol
b. Hypoxanthine aminopterin thyminine
c. Hypoxathing-guaning phosphoribosyl transferase
d. Both b and c
9. The enzymes which are commonly used in genetic engineering are
a. Exonuclease and ligase
b. Restriction endonuclease and polymerase
c. Ligase and polymerase
d. Restriction endonuclease and ligase
10. A successful hybridoma was produced by fusing
a. Plasma cells and plasmids
b. Plasma cells and myeloma cells
c. Myeloma cells and plasmids
d. Plasma cells and bacterial cells
11. The technique involved in comparing the DNA components of two samples is known as
a. Monoclonal antibody techniques
b. Genetic finger printing
c. Recombinant DNA technology
d. Polymerase chain reaction
12. Plasmids are ideal vectors for gene cloning as
a. They can be multiplied by culturing
b. They can be multiplied in the laboratory using enzymes
c. They can replicate freely outside the bacterial cell
d. They are self-replicating within the bacterial cell
13. Humans normally have 46 chromosomes in skin cells. How many autosomes would be expected in a kidney cell?
a. 46
b. 23
c. 47
d. 44
14. Pasteur effect is due to?
a. Change from aerobic to anaerobic
b. Providing oxygen to anaerobically respiring structures
c. Rapid utilization of ATP
d. Nonsynthesis of ATP
15. A mechanism that can cause a gene to move from one linkage group to another is
a. Trans location
b. Inversion
c. Crossing over
d. Duplication
16. The smallest unit of genetic material that can undergo mutation is called
a. Gene
b. Cistron
c. Replicon
d. Muton
17. The two chromatids of metaphase chrosome represent
a. Replicated chromosomes to be separated at anaphase
b. Homologous chromosomes of a diploid set
c. Non-homologous chromosomes joined at the centromere
d. Maternal and paternal chromosomes joined at the centromere
18. Malate dehydrogenase enzyme is a
a. Transferase
b. Hydrolase
c. Isomerase
d. Oxido reductase
19. In E.Coli at site is in between
a. Gal and biogenes
b. Bio and niacin genes
c. Gal and B genes
d. None of these
20. The best vector for gene cloning
a. Relaxed control plasmid
b. Stringent control plasmid
c. Both a and b
d. None of these
21. A gene that takes part in the synthesis of polypeptide is
a. Structural gene
b. Regulator gene
c. Operator gene
d. Promoter gene
22. DNA replicates during
a. G1 – phase
b. S – phase
c. G2 – phase
d. M – phase
23. A human cell containing 22 autosome and
a ‘Y’ chromosome is probably a
a. Male somatic cell
b. Zygote
c. Female somatic cell
d. Sperm cell
24. Crossing-over most commonly occurs during
a. Prophase I
b. Prophase II
c. Anaphase I
d. Telophase II
25. DNA-replication is by the mechanism of
a. Conservative
b. Semiconservative
c. Dispersive
d. None of the above
26. Production of RNA from DNA is called
a. Translation
b. RNA splicing
c. Transcription
d. Transposition
27. Nucleic acids contain
a. Alanine
b. Adenine
c. Lysine
d. Arginine
28. What are the structural units of nucleic acids?
a. N-bases
b. Nucleosides
c. Nucleotides
d. Histones
29. The most important function of a gene is to synthesize
a. Enzymes
b. Hormones
c. RNA
d. DNA
30. One of the genes present exclusively on the X-chromosome in humans is concerned with
a. Baldness
b. Red-green colour baldness
c. Facial hair/moustache in males
d. Night blindness
31. Peptide linkages are formed in between
a. Nucleotides
b. Amino acids
c. Glucose molecules
d. Sucrose
32. The nucleic acid of polio viruses is
a. DNA
b. RNA – (+) type
c. t-RNA
d. m-RNA
33. Rabies virus is
a. Nake RNA virus
b. Naked DNA virus
c. Enveloped RNA virus
d. Enveloped DNA virus
34. Example for DNA virus:
a. Polio virus b. Adeno virus
c. Echo virus d. Poty virus
35. In genetic engineering breaks in DNA are formed by enzymes known as
a. Restriction enzymes
b. Ligases
c. Nucleases
d. Hydralases
36. DNA transfer from one bacterium to another through phages is termed as
a. Transduction
b. Induction
c. Transfection
d. Infection
37. Microorganisms usually make acetyl CO-A by oxidizing
a. Acetic acid
b. Pyruvic acid
c. α-ketoglutaric acid
d. Fumaric acid
38. The method of DNA replication proposed by Watson and Crick is
a. Semi conservative
b. Conservative
c. Dispersive
d. Rolling loop
39. The distance between each turn in the helical strand of DNA is
a. 20 Ao b. 34 Ao
c. 28 Ao d. 42 Ao
40. Self-replicating, small circular DNA molecules present in bacterial cell are known
a. Plasmids
b. Cosmids
c. Plasmomeros
d. plastids
41. Western blotting is the technique used in the determination of
a. RNA
b. DNA
c. Proteins
d. All of these
42. m RNA synthesis from DNA is termed
a. Transcription
b. Transformation
c. Translation
d. Replication
43. Western blotting is a technique used in the determination of
a. DNA
b. RNA
c. Protein
d. Polysaccharides
44. Building blocks of Nucleic acids are
a. Amino acids
b. Nucleosides
c. Nucleotides
d. Nucleoproteins
45. DNA finger printing is based on
a. Repetitive sequences
b. Unique sequences
c. Amplified sequences
d. Non-coding sequences
46. The enzyme required for DNA from RNA template:
a. RNA polymerase
b. Reverse transcriptase
c. DNA polymerase
d. Terminal transferase
47. Double standard RNA is seen in
a. Reo virus
b. Rhabdo virus
c. Parvo virus
d. Retro virus
48. Example for DNA viruses:
a. Adeno virus
b. Bacteriophage T1, T2, T3, T4, T5, T6
c. Papova virus & Herpes virus and cauliflower moisaic
e. All of the above
49. The following are the RNA viruses, except
a. Reo viruses
b. Retro viruses & Bacteriophage Φ C
c. Tmv and Bacteriophages Ms2, F2
d. Dahila mosaic virus and Bacteriophages Φ × 174, M12, M13
50. The two strands of DNA are joined noncovalently by
a. Ionic bonds
b. Covalent bonds
c. Hydrogen bonds between bases
d. Polar charges
Answers
