Modern Pharmaceutical Analysis MCQs with Answers
1. Chromatography can be used to
A. form mixtures
B. change mixture compositions
C. separate mixtures into pure substances
D. all of these
2. Mobile phase can be
A. gas or liquid
B. solid or liquid
C. only solid
D. gas only
3. The fluid exiting a chromatographic column is called the
A. eluent
B. eluate
C. analyte
D. elution
4. To improve a chromatographic separation, you must
A. Increase the number of theoretical plates on the column.
B. Increase the height of the theoretical plates on the column.
C. Increase both the number and the height of the theoretical plates on the column.
D. Decrease both the number and the height of the theoretical plates on the column
5. Infrared spectroscopy provides valuable information about
A. Molecular weight
B. Melting point
C. Conjugation
D. Functional groups
6. A strong signal at 3400 cm–1
in an IR spectrum indicates the presence of
A. Alcohol
B. Ether
C. Carbonyl
D. Amine
7. In infrared spectroscopy which frequency range is known as the fingerprint region?
A. 400 – 1400cm-1
B. 1400 – 900cm-1
C. 900 – 600cm-1
D. 600 – 250cm-1
8. What is a chromophore?
A. A coloured compound
B. A group of atoms in compound responsible for electromagnetic radiation
C. A group of atoms in a compound responsible for the absorption of electromagnetic radiation
D. A group of atoms in coloured compound
9. Which of the following is an example of bulk property or general detector in HPLC
A. Fluorescence detector
B. Refractive index detector
C. Electrochemical detector
D. UV-Visible detector
10. Which of the following is used as a carrier gas in gas chromatography
A. Carbon dioxide
B. Oxygen
C. Helium
D. Methane
11. 1H nuclei located near electronegative atoms tend to be ______ relative to 1H nuclei
A. Shielded
B. Deshielded
C. Resonanced
D. Split
12. Signal splitting in NMR arises from?
A. Shielding effect
B. Spin-spin decoupling
C. Spin-spin coupling
D. Deshielding effecr
13. The base peak in mass spectrum is?
A. The lowest mass peak
B. The peak corresponding to the parent ion
C. The highest mass peak
D. The peak set to 100% relative intensity
14. Which one of the following is necessary for mass spectrometry to occur?
A. Loss of an electron
B. Change of alignment of a proton in a magnetic field
C. A molecular vibration
D. Excitation of an electron from the ground state to a higher energy state
15. Column efficiency is measured in terms of number of theoretical plates, which is:
A. Inversely related to height equivalent to theoretical plates
B. Directly related to the peak width
C. Directly related to height equivalent to theoretical plates
D. Inversely related to the peak width
16. In a chromatographic separation, which of the following is most appropriate for the qualitative identification of a substance?
A. Relative retention factor
B. Retention factor
C. Retention time
D. Resolution
17. The basis of the technique of chromatography for separating components of a mixture is?
A. the differing movement of particles of different mass in an electrical field
B. the interaction of the components with a stationary and a mobile phases
C. the absorption of infrared radiation by the components.
D. the deflection of charged particles in a magnetic field.
18. HPLC is an abbreviation for?
A. High Profit Liquid Chromatography
B. High Pressure Liquid Chromatography
C. Higher Performance Low Chromatography
D. Higher Profit Low Chromatography
19. In IR spectroscopy, the wave number for carbonyl group is observed in the range of
A. 3500-3300 cm-1
B. 2200-2100 cm-1
C. 1740-1650 cm-1
D. 3000-2800 cm-1
20. Which of the following techniques would be most useful to identify and quantify the presence of a known impurity in a drug substance?
A. NMR
B. MS
C. IR
D. HPLC
21. In mass spectrometry, fragmentation of ions is achieved through?
A. Ionization
B. Splitting
C. Solubilization
D. Coupling
22. he dipole magnetic moment (μ) is directly proportional to nuclear spin
(I), connected by a constant called the
A. Gyromagnetic ratio (γ)
B. Planck’s constant (h)
C. Nuclear susceptibility (χ)
D. Chemical shift (δ)
23. Which of the following names refers to the highest energy spin state of an ¹H nucleus in a magnetic field?
A. Spin-up
B. Spin-down
C. Parallel
D. |+½>
24. Chemical shifts (δ) are typically reported in units of
A. Gauss (G)
B. Millitesla per meter (mT/m)
C. Parts per million (ppm)
D. Percent (%)
25. What is shielding in NMR?
A. Using a curved piece of metal to block an opponent’s attack
B. Putting metal around an Rf source
C. When the magnetic moment of an atom blocks the full induced magnetic field from surrounding nuclei
D. Blocking parts of a molecule from Rf radiation
