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Microbiology & Biochemistry (Part-1 ) MCQs with Answers


1. B.anthracis was isolated by
a. Louis Pasteur
b. Robert Koch
c. Antonyvon Leewenhok
d. None of these

2. The magnefication power of electron microscope developed by Knell and Ruska is
a. 10,000x
b. 12,000x
c. 15,000x
d. 20,000x

3. Source of light in fluorescence microscopy is from
a. Mercury lamp
b. Sunlight
c. Both a and b
d. None of these

4. If 10x and 40x objectives are used air is the medium,the numerical aperture is
a. 1.5
b. 2.0
c. 1.0
d. 1.8

5. The first antibody to contact invading microorganisms was
a. IgG
b. IgM
c. IgA
d. IgD

6. According to Pasteur statements which one of the following is true
a. Living organisms discriminate between stereoisomers
b. Fermentation is a aerobic process
c. Living organisms doesn’t discriminate between stereoisomers
d. Both a and b

7. Enzymes responsible for alcoholic fermentation
a. Ketolase
b. Zymase
c. Peroxidase
d. Oxidase

8. Enhancement of virulence is known as
a. Exaltation
b. Attenuation
c. Both a and b
d. None of these

9. Term vaccine was coined by
a. Robert Koch
b. Pasteur
c. Needham
d. None of these

10. Cold like symptoms are caused by which bacteria
a. Pseudomonas
b. E.coli
c. Haemophilus influenza
d. Haemophilus streptococcus

11. In Streptococcus fecalis, the conjugation takes place at
a. Pili
b. Cell membrane
c. Cell wall
d. Flagella

12. The Baterium that is most commonly used in genetic engineering is
a. Escherichia
b. Klebsiella
c. Proteius
d. Serratia

13. The most infectious food borne disease?
a. Tetanus
b. Dysentery
c. Gas gangrene
d. Botulism

14. The percentage of alcohol used in Gram-staining is
a. 75%
b. 90%
c. 60%
d. 25%

15. Gram positive bacteria appear as
a. Pink b. Violet
c. both a & b d. None of these

16. Gram negative bacteria appear as
a. Pink
b. Violet
c. both a & b
d. None of these

17. Streptococci which are destroyed at 60°C for 30 minutes
a. Preptostreptococci
b. Strepto viridans
c. Strepto hemolyticus
d. All of these

18. Rickesia are stained with
a. Giesna and Castaneda stains
b. Macchiavello and Gimnezstains
c. Both a and b
d. Malachite green

19. Dengue fever is caused by
a. Bacteria
b. Virus
c. Fungi
d. Rickettsia

20. All of the following are energy source of bacteria except
a. Oxidation of inorganic compounds
b. Oxidation of organic compounds
c. Absorption of heat
d. Utilisation of visible light

21. What is the function of bacterial capsule?
a. Production of organism from phagocytosis
b. Helps in adherence of bacteria to surface in its environment
c. Both a and b
d. None of these

22. The bacteria, which is motile at 22oC but non-motile at 37oC is
a. Tranformation
b. Transduction
c. Conjugation
d. Cell fusion

23. The interval period between HIV infection and appearance of antibodies in serum is called
a. Intrinsic period
b. Incubation period
c. Window period
d. None of these

24. Ziehl – Neelson stain is a?
a. Simple stain
b. Counter stain
c. Differential stain
d. None of them

25. Screening test for AIDS is
a. Western blot test
b. ELISA test
c. Both a and b
d. VDRL test

26. Confirmatory test for AIDS is
a. Western blot test
b. ELISA test
c. Karpas test
d. Fujerbio test

27. A man is usually infected for tick typhus by
a. Drinking milk of sick animals
b. Tending cattle
c. Inhaling infected dust
d. All of these

28. Cholera vaccine gives protection for
a. 1 – 3 months
b. 3 – 6 months
c. 6 – 9 months
d. 9-12 months

29. Attenuation means
a. Killing of the bacteria microorganism.
b. Inactivation of bacteria
c. More activating the bacteria
d. Both A and B

30. Acid fast bacteria are
a. Neisseria
b. Staphylococci
c. Mycobacteria
d. All of the above

31. Streptolysin ‘S’ is
a. Oxygen unstable
b. Thermostable
c. Oxygen stable
d. None of these

32. The largest protozoa is
a. Balantidium coli
b. Entamoeba coli
c. Trichomonus vaginalis
d. Toxoplasma gondii

33. Mc Fadyean’s reaction used to detect
a. Bacillus anthracis
b. Brucella
c. Corynaebacterium
d. None of these

34. The motile bacteria is
a. Salmonella typhi
b. Klebsiella pneumoniae
c. Bacillus anthracis
d. Shigella flexneri

35. AIDS is caused by
a. HTLV – I
b. Bunya virus
d. All

36. How would you distinguish pseudomonas species from E-coli?
a. Gram staining
b. Morphology
c. Glucose fermentation Vs Respiration
d. All of the above

37. Hetrolactic acid bacteria produce
a. Lactic acid only
b. Lactic acid + H2O + CO2
c. Lactic acid + CO2
d. Lactic acid + alchohol + CO2

38. Staining material of gram positive bacterium is
a. Fast green
b. Haematoxylon
c. Crystal violet
d. Safranin

39. Enzyme hydrolyzing bacterial cell wall
a. Lysozome
b. Reductase
c. Protease
d. Lysozyme

40. Most molds are capable of growing in the temperature range between
a. 0o – 25oc
b.0o – 35oC
c. 10o – 25oC
d.10o – 35oC

41. Opsonin is the
a. Cellwall component
b. Plasma component
c. Serum component
d. Cytoplasm component

42. Mycobacteria are stained with
a. Gram’s staining
b. Simple staining
c. Both a and b
d. Ziehl – Neelsen’s staining

43. Bacillus Schlegelli is
a. Hydrogen – Oxydising bacteria
b. Sulphur – Oxydising bacteria
c. Iron-Oxidising bacteria
d. Nitrite oxidizing bacteria

44. The smallest virus is
a. Parvo virus
b. Rhabdo virus
c. Pox virus
d. Adeno virus

45. Cell-wall is
a. Thick in Gram positive than Gram negative
b. Thick in Gram negative than Gram positive
c. Equal in both
d. In Gram negative cell-wall is absent

46. Interferon is formed by
a. Lymphocytes
b. Lymphoblas
c. Fibroblasts
d. All of these

47. Spirochete is
a. Gonococci
b. Strphylococci
c. Treponema pallidum
d. Streptococci

48. The pigment present in red algae is
a. Rhodochrome
b. Fucoxanthin
c. Chlorophyll only
d. Chlorophyll + phycobilin

49. The anthrax disease is most frequently infected from
a. Cattle
b. Sheeps
c. Rats
d. Both a and b

50. Lysol is a
a. Sterilent
b. Disinfectant
c. Antiseptic
d. Antifungal agent


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